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Set RTS On Close
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Selecting the Set RTS On Close option will set the RTS signal on closing the port.

The Set RTS On Close option is controlled through bit 3 of byte 0 of the first DWORD of the ConfigData entry
in the FTDIPORT.INF file. This is the same entry that contains the Baud rate table.

The first DWORD is shown in bold in the INF fragment below.

[FtdiPort232.NT.HW.AddReg]  
HKR,,"ConfigData",1,01,00,3F,3F,10,27,88,13,C4,09,E2,04,71,02,38,41,9C,80,4E,C0,34,00,1A,00,0D,00,06,40,03,80,00,00,D0,80  
 

Please note that this entry is byte-reversed, so MSB the DWORD reads
3F 3F 00 01
and the least significant bit is Bit 0. When disabled (RTS not set on close), the DWORD would read 3F 3F 00 01. When enabled (RTS set on close), the DWORD would read 3F 3F 00 09.

These values are held in the registry under this key:

HKEY_LOCAL_MACHINE\SYSTEM\CurrentControlSet\Enum\FTDIBUS\{Device VID, PID and serial number}\0000\Device Parameters\ConfigData